Thiobacillus ferrooxidans tolerates Cr3+ up to 75 mM during growth on
ferrous sulphate without modification of its bacterial activity (53 mu
g protein mM(-1) Fe2+ oxidised). At pH 1.4 and in the presence of Cr2
O3, a maximal uptake capacity of 509 mg.g(-1) dry weight was obtained
with biomass harvested in the middle of the exponential growth phase.
Cr6+ uptake by T. ferrooxidans resulted in the precipitation of a chro
mium-rich compound on the surface bacterial cells, as observed by tran
smission electron microscopy.