DETECTION OF ANTI-LEISHMANIA ANTIBODIES IN BLOOD COLLECTED ON FILTER-PAPER BY THE DIRECT AGGLUTINATION-TEST

Authors
RAB MA EVANS DA
Citation
Ma. Rab et Da. Evans, DETECTION OF ANTI-LEISHMANIA ANTIBODIES IN BLOOD COLLECTED ON FILTER-PAPER BY THE DIRECT AGGLUTINATION-TEST, Transactions of the Royal Society of Tropical Medicine and Hygiene, 91(6), 1997, pp. 713-715
Citations number
20
Categorie Soggetti
Public, Environmental & Occupation Heath","Tropical Medicine
Journal title
Transactions of the Royal Society of Tropical Medicine and Hygiene → ACNP
ISSN journal
00359203
Volume
91
Issue
6
Year of publication
1997
Pages
713 - 715
Database
ISI
SICI code
0035-9203(1997)91:6<713:DOAAIB>2.0.ZU;2-U
Abstract
Human capillary blood drawn by finger pricking was absorbed on Whatman no. 4 filter paper and tested for anti-Leishmania antibodies using th e direct agglutination test (DAT). Over 1400 filter paper samples were collected from L. infantum endemic areas in northern Pakistan and com pared with 569 serum samples obtained from the same area. Eleven filte r paper samples (0.8%) gave positive results (antibody titre greater t han or equal to 1:3200), compared with 8 serum samples (1.4%); the dif ference was not statistically significant (P=0.35). The filter paper e luates compared well with their homologous sera, and were strongly cor related with respect to antibody titre (r=0.7, P<0.0001).