A STUDY OF AGING OF GOMORI ALDEHYDE FUSCHIN STAIN

The photochemical stability of Gomori Aldehyde Fuschin stain samples o f different ages was investigated by thin layer, column and high perfo rmance liquid chromatographic techniques and the component dyes isolat ed. The dyes obtained were individually and sequentially irradiated wi th simulated sunlight and the photodegradation products isolated and c haracterised by comparison of infra red spectra with those of authenti c samples. Pararosaniline was found to undergo progressive methylation leading eventually to the formation of Crystal Violet which was subse quently ethylated to Methyl Green. Methyl Green was found to undergo d isproportionation to Malachite Green. The histochemical consequences o f these reactions are discussed. (C) 1997 Elsevier Science Ltd.