ULTRASENSITIVE BIENZYME SENSOR FOR ADRENALINE

A biosensor consisting of an analyte-recycling two-enzyme system using laccase (Coriolus hirsutus) and PQQ-dependent glucose dehydrogenase i n combination with the electrochemical detection of oxygen depletion a t a platinum electrode was used for adrenaline determination in the na no-and subnanomolar concentration range. Measurements were performed i n a flow cell providing excellent baseline stability and fast recovery of the sensor. Improved design of the polymer matrix resulted in a lo wer detection limit of 200 pmol/l for adrenaline. The sensor has succe sfully been applied to the analysis of adrenaline in effluate of isola ted rabbit hearts. (C) 1997 Elsevier Science Limited.