A CHEMILUMINESCENT FIA BIOSENSOR FOR PHOSPHATE ION MONITORING USING PYRUVATE OXIDASE

We have constructed an automatic phosphate ion sensing system for the quality control of drinking water. The analyte was detected using the phosphate ion-dependent pyruvate oxidase reaction and the hydrogen per oxide produced was detected by luminol chemiluminescence catalyzed by Arthromyces ramosus peroxidase. We obtained a detection limit of 0 . 1 6 mu M phosphate ion (5 ppb phosphorus) and it was possible to detect 0 . 32 mu M phosphate ion for 48 days using pyruvate oxidase immobiliz ed on Chitopearl BCW-2601 beads. An excellent correlation (r(2) = 1 . 00) was Obtained between the results obtained using our phosphate ion sensor and those using a modified Molybdenum Blue method. (C) 1997 Els evier Science Limited.