CHEMICAL TOOLS FOR DETECTION OF INTRACELLULAR PH-CHANGE IN HALOBACTERIA

Time-dependent pH-change in halobacteria was observed with fluorescent dye. Mechanical improvement of a cell holder in a fluorescence spectr ometer enabled us to measure the intracellular pH-change that proved d ifferent from the extracellular change. Upon irradiation, the extracel lular pH of the strain initially changed into higher value and subsequ ently reverted to lower value until irradiation ceased, whereas the in tracellular pH monotonously increased for freshly prepared cells. Neve rtheless, the pH-changes in liposome with bacteriorhodopsin were compl ementary each other.