COMPARATIVE DETECTION AND QUANTITATION OF HUMAN CDK INHIBITOR MESSENGER-RNA EXPRESSION OF P15(INK4B), P16(INK4A), P16-BETA, P18(INK4C), P19(INK4D), P21(WAF1), P27(KIP1) AND P57(KIP2) BY RT-PCR USING A POLYCOMPETITIVE INTERNAL STANDARD

For comparative and quantitative analysis of human cyclin-dependent ki nase inhibitor gene expression (CKI; p15(INK4B), p16(INK4A), p16 beta, p18(INK4C), p19(INK4D), p21(WAF1), p27(KIP1) and p57(KIP2)) we set up an RT-PCR assay with a construct termed pCKIquant producing polycompe titive RNA as an internal standard. We demonstrated the reproducibilit y, accuracy and high sensitivity of the assay in the in vitro model of myeloid leukaemic HL-60 cells. We also showed that the pCKIquant CKI assay is an excellent tool for the assessment of CKI mRNA expression i n clinical samples, e.g. single cryostat sections of lymphoma biopsies .