ISOLATION OF AN ENRICHED FRACTION OF NERVE-TERMINALS FROM FISH BRAIN USING A DISCONTINUOUS PERCOLL GRADIENT

Authors
NICHOLSON RA ZHENG J
Citation
Ra. Nicholson et J. Zheng, ISOLATION OF AN ENRICHED FRACTION OF NERVE-TERMINALS FROM FISH BRAIN USING A DISCONTINUOUS PERCOLL GRADIENT, Journal of neuroscience methods, 71(2), 1997, pp. 157-162
Citations number
19
Categorie Soggetti
Neurosciences
Journal title
Journal of neuroscience methods → ACNP
ISSN journal
01650270
Volume
71
Issue
2
Year of publication
1997
Pages
157 - 162
Database
ISI
SICI code
0165-0270(1997)71:2<157:IOAEFO>2.0.ZU;2-H
Abstract
A discontinuous gradient of Percoll has been used to prepare a subcell ular fraction (fraction In) from fish brain which is greatly enriched in synaptic nerve endings (synaptosomes). Synaptosomes display well-pr eserved morphology, good functional performance as assessed by ability to release preloaded neurotransmitter following depolarization, and t he fraction as a whole has an enzyme profile consistent with the prese nce of nerve endings. The procedure is rapid, highly reliable and prov ides a mean yield of 4.5 mg synaptosomes per g wet weight of brain. (C ) 1997 Elsevier Science B.V.