ADSORPTION OF ALPHA-AMYLASE ON DEXTRIN IMMOBILIZED ON KIESELGUHR OR CHITIN

Dextrin prepared from corn starch was immobilized on kieselguhr or chi tin using a crosslinking reagent (epichlorohydrin), to produce a new a dsorbent having a high affinity for alpha-amylase. The dextrins were i mmobilized at levels of 300-340 and 200-270 mg/g adsorbent on chitin a nd kieselguhr, respectively. There was an optimum crosslinking time fo r alpha-amylase adsorption because of a balance between the number of adsorption sites and the affinity of the immobilized dextrin for alpha -amylase. Adsorption of alpha-amylase on the crosslinked immobilized d extrin was as much as 50 times higher than the value on raw corn starc h. When expressed in terms of the content the adsorption of alpha-amyl ase varied with the type of support (kieselguhr and chitin) because of the different state of the immobilized dextrin. (C) 1997 Published by Elsevier Science Ltd. All rights reserved.