SPINONIN, A NOVEL GLYCOSIDE FROM ONONIS-SPINOSA SUBSP LEIOSPERMA

The roots of Ononis spinosa subsp. leiosperma (Leguminosae) afforded a new glycoside, spinonin (1), possessing a novel skeleton, in addition to the known isoflavonoid glycoside, ononin [7 beta-(glucosyloxy)form ononetin] (2) and the known pterocarpan, 7-demethoxy-7-D-(glucosyloxy) -homopterocarpin (3). The structure of the new isolate was elucidated by spectral methods including H-1 and C-13 NMR, COSY, APT, HETCOR, HMB C, NOESY, CD, FABMS, HRMS, EIMS, CIMS, and some chemical reactions. Sp inonin was inactive against a number of human cancer cell Lines and HI V-1 reverse transcriptase. The compounds 1 and 3 showed weak activity against Pseudomonas aeruginosa, whereas 2 was active against beta-hemo lytic Streptococcus.