NOVEL PHOSPHODIESTERASE-4 INHIBITOR T-440 REVERSES AND PREVENTS HUMANBRONCHIAL CONTRACTION INDUCED BY ALLERGEN

To study the roles of phosphodiesterase (PDE) 4 in the human airways, we examined the effect of the novel PDE4 inhibitor T-440 in the isolat ed human bronchus. T-440 inhibited PDE4 extracted from human bronchial smooth muscle. IC50 values for the effect of T-440, rolipram (a PDE4 inhibitor) and theophylline on PDE4 activity of the bronchial tissues were 0.08 mu M, 2 mu M and >100 mu M, respectively. T-440 (10(-6) M to 10(-5) M) and aminophylline (3.3 X 10(-5) M) significantly reversed t he 10(-5) M histamine-induced contraction, the efficacy of 10(-6) M T- 440 being almost the same as that of 3.3 X 10(-5) M aminophylline. T-4 40 (10(-6) M to 10(-5) M) and aminophylline (3.3 X 10(-5) M) significa ntly reversed the 10(-4) M ACh-induced contraction. But their reversal effects on the ACh-induced contraction were weaker than those on the histamine-induced contraction. T-440 (10(-5) M) significantly reversed the contraction induced by allergen in passively sensitized bronchi. The efficacy of the reversal effect of T-440 (10(-5) M) was significan tly higher than that of aminophylline (10(-5) M). T-440 and aminophyll ine significantly relaxed the basal tension, but pretreatment with T-4 40 or aminophylline did not significantly prevent histamine- or ACh-in duced contraction. In contrast, both T-440 (10(-5) M) and aminophyllin e (3.3 X 10(-5) M) prevented the contraction induced by allergen, whic h suggests that PDE4 inhibitor inhibits the release of chemical mediat ors probably from bronchial mast cells in the allergic response. T-440 (10(-6) M to 10(-5) M) caused the accumulation of cAMP at the concent ration that relaxed histamine-induced contraction. Thus selective PDE4 inhibitor is a candidate for the treatment of asthma.