CDNA CLONING AND GENE-EXPRESSION OF PHENYLALANINE AMMONIA-LYASE IN LITHOSPERMUM-ERYTHRORHIZON

Citation
K. Yazaki et al., CDNA CLONING AND GENE-EXPRESSION OF PHENYLALANINE AMMONIA-LYASE IN LITHOSPERMUM-ERYTHRORHIZON, Bioscience, biotechnology, and biochemistry, 61(12), 1997, pp. 1995-2003
Citations number
35
ISSN journal
09168451
Volume
61
Issue
12
Year of publication
1997
Pages
1995 - 2003
Database
ISI
SICI code
0916-8451(1997)61:12<1995:CCAGOP>2.0.ZU;2-A
Abstract
Two cDNA clones (LEPAL-1 and LEPAL-2) encoding phenylalanine ammonia-l yase were isolated from cell suspension cultures of Lithospermum eryth rorhizon. Northern kinetic studies showed that LEPAL-1 mRNA contents m arkedly increased one day after inoculation of the cells into fresh me dium, then decreased to the steady-state level. The course of mRNA acc umulation paralleled that of PAL enzyme activity. The rapid induction of PAL activity seems to reflect the induction of dihydroechinofuran b iosynthesis, while shikonin was produced at the steady-state level of PAL activity. The course of LEPAL-2 mRNA accumulation seemed to be sim ilar to, but much lower than that of LEPAL-1. In the intact plant, bot h genes are expressed mainly in the root, the organ in which shikonin is exclusively produced and accumulated. Genomic Southern blot analyse s showed that both genes are present in the genome as single copies.