PRODUCTION OF NEW RESTRICTION-ENDONUCLEASE VPAK11BI RECOGNIZING SEQUENCE 5'-GGWCC-3' IN A HEMOLYSIN-LESS MUTANT OF VIBRIO-PARAHAEMOLYTICUS

Authors
UENO H KITA A MIYAHARA M ISHIWATA N MISE K KATO I ISHINO Y
Citation
H. Ueno et al., PRODUCTION OF NEW RESTRICTION-ENDONUCLEASE VPAK11BI RECOGNIZING SEQUENCE 5'-GGWCC-3' IN A HEMOLYSIN-LESS MUTANT OF VIBRIO-PARAHAEMOLYTICUS, Bioscience, biotechnology, and biochemistry, 61(12), 1997, pp. 2129-2130
Citations number
14
Journal title
Bioscience, biotechnology, and biochemistry → ACNP
ISSN journal
09168451
Volume
61
Issue
12
Year of publication
1997
Pages
2129 - 2130
Database
ISI
SICI code
0916-8451(1997)61:12<2129:PONRVR>2.0.ZU;2-Z
Abstract
High restriction endonuclease activity was found in a haemolysin-less mutant of the Vibrio parahaemolyticus 1743-1 strain. The endonuclease, named VpaK11BI, recognized the palindromic pentanucleotide sequence o f 5'-GGWCC-3' and cleaved double-stranded DNA after the first G, which is exactly the same as the specificity of AvaII. The haemolysin-less mutant of V. parahaemolyticus is now available for producing the valua ble restriction endonuclease on a commercial scale.