MUTATIONAL ANALYSIS OF THE POTATO-VIRUS-Y 5'-UNTRANSLATED REGION FOR ALTERATION IN TRANSLATIONAL ENHANCEMENT IN TOBACCO PROTOPLASTS

Authors
YANG LJ HIDAKA M SONODA J MASAKI H UOZUMI T
Citation
Lj. Yang et al., MUTATIONAL ANALYSIS OF THE POTATO-VIRUS-Y 5'-UNTRANSLATED REGION FOR ALTERATION IN TRANSLATIONAL ENHANCEMENT IN TOBACCO PROTOPLASTS, Bioscience, biotechnology, and biochemistry, 61(12), 1997, pp. 2131-2133
Citations number
13
Journal title
Bioscience, biotechnology, and biochemistry → ACNP
ISSN journal
09168451
Volume
61
Issue
12
Year of publication
1997
Pages
2131 - 2133
Database
ISI
SICI code
0916-8451(1997)61:12<2131:MAOTP5>2.0.ZU;2-2
Abstract
The 185 nucleotide 5' untranslated region (5'UTR) of potato virus Y or dinary strain (PVY-O) showed translation-enhancing activity on the bet a-glucuronidase (GUS) gene in tobacco protoplasts. Mutational analysis of the 5'UTR was done to find sequence motifs necessary for the enhan cement. Deletions within the 1-130 nucleotide region of 5'UTR stimulat ed the GUS expression in some cases, while the GUS activity declined w ith deletions in the 131-185 nucleotide region. The results indicated that the last 55 nucleotides of PVY-O 5'UTR might play the much import ant role in the translational enhancement in plant cells.