SUBSTANTIALLY COMPLETE REMOVAL OF 3 MAJOR ALLERGENIC SOYBEAN PROTEINS(GLY-M BD 30K, GLY-M BD 28K, AND THE ALPHA-SUBUNIT OF CONGLYCININ) FROM SOY PROTEIN BY USING A MUTANT SOYBEAN, TOHOKU-124

A wild-type soybean contains three major allergenic proteins, Gly m Bd 30K, the alpha-subunit of conglycinin, and Gly m Bd 28K. A geneticall y mutated soybean (Tohoku 124), which was originally developed as a cu ltivar lacking the alpha- and alpha'-subunits of conglycinin, was also found to lack Gly m Bd 28K from immunoblot analysis using monoclonal antibodies specific to Gly m Bd 28K. This finding indicates the possib ility to prepare soy milk and soy proteins containing none of the thre e major allergenic soybean proteins from this cultivar. By applying th e previous removal procedure [Samoto et al., Biosci. Biotech. Biochem. , 60, 1911-1913 (1996)] to Tohoku 124, the substantially complete remo val of the three major allergenic proteins from the soy milk was attai ned. The removal rates of Gly m Bd 30K, alpha-subunit of conglycinin, and Gly m Bd 28K were 99.8, 100, and 100%, respectively.