IDENTIFICATION OF LACTIC-ACID BACTERIA ISOLATED FROM RONCAL AND IDIAZABAL CHEESES

The lactic flora of two ewe's-milk cheeses regulated by Appellations o f Origin, Ron cal and Idiazabal cheeses, manufactured in the autonomou s region of Navarre (Spain), was identified and compared. 263, 494, an d 464 isolates of lactococci, lactobacilli, and leuconostocs, respecti vely, were isolated throughout ripening from both cheeses and presumab ly identified using morphological, phenotypical and biochemical method s. The distribution of lactococci species was not significantly differ ent in the two cheeses, Lactococcus lactis subsp lactis being the main species from 10 days of ripening. Lactobacillus casei and Lactobacill us plantarum were the major lactobacilli species in both cheeses. In R oncal cheese L casei accounted for 53% of the isolates and L plantarum for 30%, whereas in Idiazabal cheese the frequency oft casei (39%) wa s slightly lower than the frequency of L plantarum (44%). Leuconostoc mesenteroides subsp mesenteroides and Leuconostoc mesenteroides subsp dextranicum were the predominant leuconostoc Species in both cheeses, but the ratios of the two subspecies were approximately 3:1 in the Ron cal cheese and closer to 1:1 in the Idiazabal cheese. The experimental results of this work are considered to be a first step to prepare ind igenous starters including representative bacteria isolated from each type of cheese.