EFFECT OF TEMPERATURE, FILTER-PAPER AND C ULTURE MEDIA ON PHYSIOLOGY OF MACROPHOMINA-PHASEOLINA

The effect of temperature, filter paper and culture media on mycelial growth, fertility, picnidia production, and sporulation of Macrophomin a phaseolina ''in vitro'' were studied The fungus was submited to diff erent temperatures (15 degrees, 20 degrees, 25 degrees, 30 degrees and 35 degrees C), on PDA and soybean-agar, with and without filter paper overposition, under continuous light, during seven days. The evaluati on consisted on determination of the colony diameter, number of steril e picnidia and fertility, and conidial concentration for each treatmen t. The results showed that the culture media used was favourable to th e mycelial growth, except at 15oC. The major production of picnidia oc curred in soybean-agar interaction with filter paper at 35 degrees C; however, to fertility of picnidia, the interaction PDA with filter pap er at 25 degrees C showed better condition. For conidia production, th e medium soybean-agar with filter paper at 30 degrees C was more favou rable.