IDENTIFICATION OF THE TYKY HOMOLOGOUS SUBUNIT OF COMPLEX-I FROM NEUROSPORA-CRASSA

Authors
DUARTE M SCHULTE U VIDEIRA A
Citation
M. Duarte et al., IDENTIFICATION OF THE TYKY HOMOLOGOUS SUBUNIT OF COMPLEX-I FROM NEUROSPORA-CRASSA, Biochimica et biophysica acta. Bioenergetics, 1322(2-3), 1997, pp. 237-241
Citations number
37
Journal title
Biochimica et biophysica acta. Bioenergetics → ACNP
ISSN journal
00052728
Volume
1322
Issue
2-3
Year of publication
1997
Pages
237 - 241
Database
ISI
SICI code
0005-2728(1997)1322:2-3<237:IOTTHS>2.0.ZU;2-V
Abstract
A polypeptide subunit of complex I from Neurospora crassa, homologous to bovine TYKY, was expressed in Escherichia coli, purified and used f or the production of rabbit antiserum. The mature mitochondrial protei n displays a molecular mass of 21280 Da and results from cleavage of a presequence consisting of the first 34 N-terminal amino acids of the precursor. This protein was found closely associated with the peripher al arm of complex I. (C) 1997 Elsevier Science B.V.