K. Subhash et al., EMS-INDUCED LINCOMYCIN RESISTANCE IN RED-PEPPER (CAPSICUM-ANNUUM L.), In vitro cellular & developmental biology. Plant, 33(4), 1997, pp. 285-287
Ethyl methane sulphonate (EMS) is a potential mutagen to induce lincom
ycin resistance in Capsicum annuum. Mutagenized cotyledons were cultur
ed on shoot regenerating medium containing lincomycin (100 mgl(-1)). A
pproximately 14% of regenerated shoots were chlorophyll deficient and
about 4% of regenerated shoots were green from mutaganized cotyledons.
The regenerated green plants were resistant to lincomycin but sensiti
ve to chloramphenicol, kanamycin, spectinomycin, and streptomycin. Rec
iprocal crosses were made between resistant and sensitive plants. Inhe
ritance of lincomycin resistance was transmitted as a non-Mendelian tr
ait. Lincomycin resistance is a first selectable and maternally inheri
ted organelle encoded genetic marker described in chili pepper. Such m
utants should be useful in designing biochemical selection schemes to
recover somatic hybrids and cybrids.