BIOTIN-LABELED DNA-PROBE IN A PCR-BASED ASSAY INCREASES DETECTION SENSITIVITY FOR THE EQUINE HEMOPARASITE BABESIA-CABALLI

Authors
SAHAGUNRUIZ A WAGHELA SD HOLMAN PJ CHIEVES LP WAGNER GG
Citation
A. Sahagunruiz et al., BIOTIN-LABELED DNA-PROBE IN A PCR-BASED ASSAY INCREASES DETECTION SENSITIVITY FOR THE EQUINE HEMOPARASITE BABESIA-CABALLI, Veterinary parasitology, 73(1-2), 1997, pp. 53-63
Citations number
27
Categorie Soggetti
Parasitiology,"Veterinary Sciences
Journal title
Veterinary parasitology → ACNP
ISSN journal
03044017
Volume
73
Issue
1-2
Year of publication
1997
Pages
53 - 63
Database
ISI
SICI code
0304-4017(1997)73:1-2<53:BDIAPA>2.0.ZU;2-B
Abstract
wA DNA probe from Babesia caballi (Bcl) was selected by antibody scree ning of a genomic library. The Eel probe hybridized specifically to B. caballi genomic DNA. A polymerase-chain-reaction-based assay for B. c aballi DNA was developed from primers deduced from the probe nucleotid e sequence. An amplified product of 1.6 kb was detected from as little as 500 fg B. caballi template DNA. Sensitivity increased 1000-fold wh en the biotin-labeled Eel probe was hybridized to the amplicons in a S outhern blot. (C) 1997 Elsevier Science B.V.