SIMULATION OF TATA BOX SEQUENCES IN EUKARYOTES

We studied nucleotide sequences of TATA box in eukaryotic genes and th eir affinity to TATA-binding protein (TBP). The DNA affinity to TBP de pended on the concentration of dinucleotides TA, TG, TC, AA, and AG. W e proposed a method for predicting the DNA affinity to TBP based on it s sequence, and applied it to promoters of eukaryotic genes, We observ ed the only peak of high affinity to TBP at position -30, which is the optimal TATA box position. Promoter sequences around the TATA box pro ved to have lower affinity to TBP than the arbitrary sequences. We sim ulated DNA sequences with high central and low terminal affinity to TB P, and showed reliable similarity between the simulation and the TATA boxes of eukaryotic genes.