COMPUTER CONFORMATION ANALYSIS OF TATA-BOX SEQUENCES IN EUKARYOTIC PROMOTERS

We have developed a method for revealing local conformation properties of binding sites for transcription factors, based on conformation ind ices of B-sheet DNA hexanucleotide duplexes and computer system SITEVI DEO. Analysis of sequences with TATA box has revealed the promoter reg ions with certain conformation indices of B-sheet DNA significantly ch anged as compared with arbitrary sequences: TATA-box DNA has a lower a ngle of helical twist, smaller distance between neighbor base pairs al ong the DNA helix axis, wider minor and narrower major DNA grooves.