G. Yucel et al., INCREASED RESISTANCE TO OXIDATIVE STRESS IN NORMAL AND GLUCOSE-6-PHOSPHATE DEHYDROGENASE-DEFICIENT HEMOLYSATES IN THE PRESENCE OF ENZYME SUBSTRATES, International journal of clinical & laboratory research, 27(1), 1997, pp. 55-59
Erythrocytes and hemolysates from 10 normal and 10 glucose-6-phosphate
dehydrogenase-deficient individuals were incubated with cumene hydrop
eroxide, and free radical-induced lipid peroxidation was monitored by
chemiluminescence. Chemiluminescence intensities in erythrocytes of no
rmal and deficient subjects were similar in the presence or absence of
glucose-6-phosphate dehydrogenase substrates. Hemolysates of normal a
nd deficient subjects also showed similar chemiluminescence in the abs
ence of substrates. However, with the addition of substrates to the in
cubation medium, deficient hemolysates reached maximum chemiluminescen
ce intensity within a shorter period, and maximum values were higher t
han in normal hemolysates. We believe this offers a new means of detec
tion of glucose-6-phosphate dehydrogenase-deficient patients.