PASSIVE IMMUNE PROTECTION OF TURKEYS AGAINST CORYZA

Antisera made against whole cells of Bordetella avium protected turkey s against disease signs of turkey coryza, but antiserum against the de rmonecrotic heat-labile toxin (DHLT) did not. Neither antiserum agains t whole cells nor antiserum against DHLT protected turkeys against col onization of the trachea by B. avium. At least 25 bands in whole cell lysate of B. avium separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) reacted in immunoblots with protective antiserum. These bands occurred at <40 kDa and >105.3 kDa. DHLT had an isoelectric point (pI) in the range pH 6.3-6.7. Following SDS-PAGE of isoelectric-focused fractions, two bands were recognized by anti-DHLT with immunoblots of pI 6.3, pI 6.5, and pI 6.7 fractions separated by SDS-PAGE.