FRESH VS AGED BENZYLPENICILLIN ON NON-IGE RESPONSES IN MICE

AIM: To study whether or not the freshly prepared benzylpenicillin cou ld induce different non-IgE antibody response from aged benzylpenicill in. METHODS: Antibody response was determined by enzyme-linked immunos orbent assay (ELISA). Antigen molecules recognized by antibodies and a ntigenic cross reactions were tested by hapten inhibition assay. RESUL TS: Isotypes of specific non-IgE antibodies induced by freshly prepare d benzylpenicillin were mainly IgM, and then IgG and IgA. Some parts o f specific antibodies recognized benzylpenicillin molecule and major p arts combined with degraded or transforming products. Isotypes of anti bodies responsible for cross reaction were mainly IgG between benzylpe nicillin and ampicillin and IgM between benzylpenicillin and piperacil lin. CONCLUSION: Freshly prepared and aged benzylpenicillin induced di fferent non-IgE antibody response.