REDUCTION OF LIPID-PEROXIDATION IN DIFFERENT BRAIN-REGIONS BY A COMBINATION OF ALPHA-TOCOPHEROL AND ASCORBIC-ACID

Lipid peroxidation an outcome of free radicals activity has been hypot hesized as one of the possible factor involved in the pathogenesis of neuronal damage. We investigated the effects of free radical scavenger s, alpha-tocopherol (T) and ascorbic acid (A) combination (TA-combinat ion) to attenuate tert-butyl hydroperoxide (t-BuOOH)-induced lipid per oxidation in different regions of mice brain. Examinations of effect o f three regimens (100, 200, 300 mg/kg body weight) of t-BuOOH on mid b rain, cerebellum, striatum, cortex and hippocampus revealed dose and t ime dependent increase in lipid peroxidation. We observed that prior s upplementation of TA-combination reduced lipid peroxidation induced by t-BuOOH in every brain region. These findings suggest that TA-combina tion may play a vital role in protecting the brain tissue against free radicals.