SPECIFICITY OF MUTAGENESIS BY 4-AMINOBIPHENYL - MUTATIONS AT G-RESIDUES IN BACTERIOPHAGE-M13 DNA AND G-]C TRANSVERSIONS AT A UNIQUE DG(8-ABP) LESION IN SINGLE-STRANDED-DNA

Mutagenesis by the human bladder carcinogen 4-aminobiphenyl (ABP) was studied in single-stranded DNA from a bacteriophage M13 cloning vector . In comparison to ABP lesions in double-stranded DNA, Lesions in sing le-stranded DNA were similar to 70-fold more mutagenic and 50-fold mor e genotoxic. Sequencing analysis of ABP-induced mutations in the lacZ gene revealed exclusively base-pair substitutions, with over 80% of th e mutations occurring at G sites; the G at position 6310 accounted for 25% of the observed mutations. Among the sequence changes at G sites, G-->T transversions predominated, followed by G-->C transversions and G-->A transitions. In order to further elucidate the mutagenic mechan ism of ABP, an oligonucleotide containing the major DNA adduct, N-(deo xyguanosin-8-yl)-4-aminobiphenyl (dG(8-ABP)), was situated within the PstI site of a single-stranded M13 genome. After in vivo replication o f the adduct containing ABP-modified and control (unadducted) genomes, the mutational frequency and mutational specificity of the dG(8-ABP) lesion were determined, The targeted mutational efficiency was similar to 0.01 %, and the primary mutation observed was the G-->C transversi on. Thus dG(8-ABP), albeit weakly mutagenic at the PstI site, can cont ribute to the mutational spectrum of ABP lesions.