PREVALENCE OF COLD-TOLERANT CLOSTRIDIA ASSOCIATED WITH VACUUM-PACKED BEEF AND LAMB STORED AT ABUSIVE AND CHILL TEMPERATURES

A survey was conducted for the presence of cold-tolerant (i.e. capable of growing at less than 5 degrees C) Clostridium spp, in fresh vacuum -packed beef and lamb obtained from nine export meat plants. Prevalenc e was determined before and after storage at abusive (15 degrees C) an d non-abusive (-1.5 degrees C) temperatures, and instances of pack fai lure by ''blowing'' were recorded. Overall, only three samples (6.7%) were positive for the presence of this group of micro-organisms on ini tial testing. After storage at 15 degrees C for 8 weeks, cold-tolerant clostridia were found in 51.1% of samples, all of which had ''blown'' . After storage at -1.5 degrees C for 12 weeks, only 2 samples (4.4%) accumulated significant amounts of gas, and both contained cold-tolera nt clostridia. With vacuum-packed product stored at 15 degrees C, appr oximately 29% of the clostridial strains isolated at 15 degrees C were cold-tolerant species capable of growing at less than 5 degrees C. Ho wever, none of them were able to grow at -1.5 degrees C. Only psychrop hilic and psychrotrophic clostridia were isolated from fresh samples a nd those stored at -1.5 degrees C.