SYNTHESIS AND DNA DUPLEX STABILITIES OF OLIGONUCLEOTIDES CONTAINING C-5-(3-METHOXYPROPYNYL)-2'-DEOXYURIDINE RESIDUES

Authors
TOLSTIKOV VV STETSENKO DA POTAPOV VK SVERDLOV ED
Citation
Vv. Tolstikov et al., SYNTHESIS AND DNA DUPLEX STABILITIES OF OLIGONUCLEOTIDES CONTAINING C-5-(3-METHOXYPROPYNYL)-2'-DEOXYURIDINE RESIDUES, Nucleosides & nucleotides, 16(3), 1997, pp. 215-225
Citations number
26
Categorie Soggetti
Biology
Journal title
Nucleosides & nucleotides → ACNP
ISSN journal
07328311
Volume
16
Issue
3
Year of publication
1997
Pages
215 - 225
Database
ISI
SICI code
0732-8311(1997)16:3<215:SADDSO>2.0.ZU;2-A
Abstract
ethoxytrityl-5-(3-methoxypropynyl)-2'-deoxyuridine phosphoroamidite wa s synthesized with the use of commercial 3-methoxypropyne. Oligonucleo tides (ODNs) containing 5-(3-methoxypropynyl)-2'-deoxyuridine in diffe rent positions were prepared. The stabilities of the duplexes formed b y these ODNs with the complementary templates are increased in compari son with the unmodified counterparts. On average modified residue inco rporated, the Tm is raised by 1 degrees C.