ASYMMETRIC POLYMERASE CHAIN-REACTION IMPROVES STREPTAVIDIN-BIOTIN BASED PURIFICATION OF POLYMERASE CHAIN-REACTION PRODUCTS PRIOR TO MATRIX-ASSISTED-LASER-DESORPTION IONIZATION TIME-OF-FLIGHT MASS-SPECTROMETRICANALYSIS/

Authors
JURINKE C VANDENBOOM D KOSTER H
Citation
C. Jurinke et al., ASYMMETRIC POLYMERASE CHAIN-REACTION IMPROVES STREPTAVIDIN-BIOTIN BASED PURIFICATION OF POLYMERASE CHAIN-REACTION PRODUCTS PRIOR TO MATRIX-ASSISTED-LASER-DESORPTION IONIZATION TIME-OF-FLIGHT MASS-SPECTROMETRICANALYSIS/, Rapid communications in mass spectrometry, 12(1), 1998, pp. 50-52
Citations number
14
Categorie Soggetti
Spectroscopy,"Chemistry Analytical
Journal title
Rapid communications in mass spectrometry → ACNP
ISSN journal
09514198
Volume
12
Issue
1
Year of publication
1998
Pages
50 - 52
Database
ISI
SICI code
0951-4198(1998)12:1<50:APCISB>2.0.ZU;2-A
Abstract
Asymmetric polymerase chain reaction (PCR) simplifies the application of biotin-streptavidin based purification formats for PCR products in matrix-assisted laser desorption/ionization time-of-flight mass spectr ometric analysis. The use of an excess of non-biotinylated primer impr oves the turnover of biotinylated primer. Thereby performance deficien cies caused by non-extended biotinylated primer are eliminated. (C) 19 98 John Wiley & Sons, Ltd.