SIMULTANEOUS DETERMINATION OF CYSTEINE SULFINIC ACID AND HYPOTAURINE IN RAT-TISSUES BY COLUMN-SWITCHING HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH ELECTROCHEMICAL DETECTION

Cysteine sulfinic acid (CSA) and hypotaurine (HT) were determined by e lectrochemical detection with a glassy carbon electrode at 0.95 V vs. Ag/AgCl. The separation of CSA and HT was accomplished by coupled-colu mn liquid chromatography, consisting of an anion-exchange column and a cation-exchange column. For the determination of CSA and HT in rat ti ssues, a column-switching system was introduced to remove interference s from late-eluting endogenous substances. The limits of determination were 0.05 mu M for both sulfinic acids. The average precisions (C.V.) over the concentration range of 0.05 to 5 mu M were 4.3% for CSA and 4.2% for HT. (C) 1997 Elsevier Science B.V.