SIMPLE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC METHOD FOR DETERMINATION OF LOSARTAN AND E-3174 METABOLITE IN HUMAN PLASMA, URINE AND DIALYSATE

A simple high-performance liquid chromatographic (HPLC) method was dev eloped for the determination of losartan and its E-3174 metabolite in human plasma, urine and dialysate. For plasma, a gradient mobile phase consisting of 25 mM potassium phosphate and acetonitrile pH 2.2 was u sed with a phenyl analytical column and fluorescence detection. For ur ine and dialysate, an isocratic mobile phase consisting of 25 mM potas sium phosphate and acetonitrile (60:40, v/v) pH 2.2 was used. The meth od demonstrated linearity from 10 to 1000 ng/ml with a detection limit of 1 ng/ml for losartan and E-3174 using 10 mu l of prepared plasma, urine or dialysate. The method was utilized in a study evaluating the pharmacokinetic and pharmacodynamic effects of losartan in patients wi th kidney failure undergoing continuous ambulatory peritoneal dialysis (CAPD). (C) 1997 Elsevier Science B.V.