Do. Krause et al., RIBOTYPING OF ADHERENT LACTOBACILLUS FROM WEANING PIGS - A BASIS FOR PROBIOTIC SELECTION BASED ON DIET AND GUT COMPARTMENT, Anaerobe, 3(5), 1997, pp. 317-325
Dietary inoculates for weaned pigs often include lacobacilli, but the
effects of age and diet on the ecology of adherent lactobacilli are no
t well known. Four experimental treatments included 16 newborn piglets
: a pre-weaning treatment (PW) included four pigs that were sacrificed
at 28 days of age; a sow treatment (Sow) had four pigs that remained
with the sow (Sow) from 28 to 38 days of age and were sacrificed at 38
days of age; and two treatments in which four pigs each, were placed
on a corn-soy diet with (CSL), or without (CS) 40% lactose. Adherent l
actobacilli were isolated from the pars esophagus, ileum, and cecum, a
nd characterised phenotypically. A 23S rDNA probe hydrized to EcoRI or
PstI digested DNA, and a 74.8% cutoff individualize isolates into 36
clusters. The most predominant strains found in the Sow, CSL, and CS t
reatments were the same, but different strains predominated in the PW
animals. The most predominant strains found in the pars esophagus and
ileum were the same, but different strains predominated in the cecum.
(C) 1997 Academic Press.