Ga. Friedrich et al., THE SECRETORY PROTEIN SEC8 IS REQUIRED FOR PARAXIAL MESODERM FORMATION IN THE MOUSE, Developmental biology, 192(2), 1997, pp. 364-374
The sec8 gene, isolated in a gene trap screen in embryonic stem cells,
is required for paraxial mesoderm formation in the mouse. Homozygous
sec8 mutant embryos initiate gastrulation but are unable to progress b
eyond the primitive streak stage and die shortly afterward. The genomi
c locus and cDNA of the sec8 gene have been cloned. An open reading fr
ame in the cDNA encodes a 971-amino-acid leucine-rich protein, similar
to rat rSec8. A description of the mutant phenotype and the cloning o
f the gene is presented here and the results are considered in light o
f the possibility that the Sec8 protein is involved in secretion. (C)
1997 Academic Press.