RELATIONSHIP BETWEEN AIRWAY MICROVASCULAR LEAKAGE, EDEMA, AND BASE-LINE AIRWAY FUNCTIONS

This study was designed to examine the relationship among microvascula r leakage, edema, and baseline airway function. Microvascular leakage was induced in the airways of anesthetized, tracheostomized New Zealan d White rabbits (n = 22) by using nebulized N-formyl-methionyl-leucyl- phenylalanine (10 mg) and was measured in the trachea by using the Eva ns blue dye technique. Airway wall thickness was assessed morphometric ally in the right main bronchus after Formalin fixation at a pressure of 25 cmH(2)O. Areas calculated included the mucosal wall area, the ad ventitial wall area, the total wall area, and the percentage of total wall area consisting of blood vessels. A neutrophil count was also per formed by analyzing numbers of cells in both the mucosal wall area and the adventitial wall area. Airway function was assessed before and 30 min after challenge with N-formyl-methionyl-leucyl-phenylalanine by d etermining airway resistance, functional residual capacity, specific a irway resistance, and flow-volume and pressure-volume curves (after pa ralysis of the animals with suxamethonium). The concentration of Evans blue dye in tracheal tissue ranged from 31.3 to 131.2 mu g. There was a significant correlation between this concentration and both the adv entitial wall area (P < 0.01) and mucosal neutrophil numbers (P < 0.00 5). There was no correlation between Evans blue concentration and eith er blood vessel area or changes in respiratory physiology parameters b efore and after challenge. There was no significant difference between any respiratory physiology measurements before and after challenge. W e conclude that an increase in microvascular leakage correlates with a irway edema in the adventitia; however, these airway changes have no s ignificant effect on airway elastic or resistive properties.