T. Murakata et al., IMMOBILIZATION OF LIPASE WITH POLYION COMPLEX AND ITS ESTERIFICATION ACTIVITY IN ORGANIC MEDIUM, Journal of Chemical Engineering of Japan, 30(2), 1997, pp. 195-201
A novel method to immobilize lipase on inorganic particles or on an in
organic porous plate is proposed. The method consists of entrapment of
lipase into polyion complex, followed by fixation of the complex on t
he inorganic supports. It was simpler and offered much saving in time,
comparing with conventional entrapping and covalently binding methods
. Both the lipases immobilized on the particles and the plate showed h
igh catalytic activity for esterification in organic medium, and the a
ctivity depended on kinds of polyion complex and water contents in the
immobilized-lipases. The immobilized-lipases sustained their activity
up to a significantly high temperature of 60 degrees C, which suggest
s that lipase and polyion complex interact to enhance thermal stabilit
y of lipase. Activity holding with repeated uses was also studied.