Mw. Frohlich et Em. Meyerowitz, THE SEARCH FOR FLOWER HOMEOTIC GENE HOMOLOGS IN BASAL ANGIOSPERMS ANDGNETALES - A POTENTIAL NEW SOURCE OF DATA ON THE EVOLUTIONARY ORIGIN OF FLOWERS, International journal of plant sciences, 158(6), 1997, pp. 131-142
The evolutionary origin of flowering plants has long been contentious.
The large morphological gap between flowering plants and their potent
ial gymnosperm relatives makes homology difficult to assess. Uncertain
ty at the base of the angiosperm clade prevents firm reconstruction of
plesiomorphic flower characters. The recent discovery of homeotic gen
es that specify flowers and flower organs raises the possibility of a
new class of evidence bearing on flower origins. Homeotic genes may gi
ve strong evidence on homology. Sequence changes or events related to
morphological evolution may help resolve the base of the flowering pla
nt tree. This article reports the creation of resources to facilitate
isolation of homeotic and other genes from taxa critical to flowering
plant origins: we have made 16 genomic DNA libraries of 15 species, in
cluding Gnetales (Welwitschia [two libraries], Gnetum [two species], a
nd Ephedra) and basal angiosperms (Nymphaea, Peperomia, Magnolia, Illi
cium, Drimys, Cinnamomum, Trochodendron, and Platanus), as well as an
advanced monocot (Juncus) and two species of the advanced dicot Heliot
ropium. Sequences of the first genes cloned from these libraries, a LE
AFY homolog from Welwitschia and one from Gnetum, along with recently
released pine sequences, demonstrate that a paralogous duplication of
LEAFY predated the divergence of Coniferales and Gnetales. LEAFY is no
t always single copy in diploids but has persisted as paralogs for a l
ong interval.