HYDROLYSIS OF WATER-SOLUBLE CELLULOSE-ACETATE PREPARED FROM WASTE NEWSPAPERS BY IMMOBILIZED CELLULASE

For the purpose of saccharifying waste newspapers by immobilized enzym e, the waste newspapers were delignified by cooking them with acetic a cid or oxygen-alkali and then partially acetylated in non-aqueous solv ent. This treatment converted almost completely the polysaccharide fra ction of the the delignified waste newspapers into water-soluble mater ial. This substrate was hydrolyzed by the dual reaction system with th e immobilized enzyme prepared from commercial cellulase. The couplings of cellulase on two carriers, DEAE-Toyopearl and Chitopearl were inve stigated. The recoveries of the maximum CMCase activity by each carrie r were 54 and 42%, respectively. The immobilization caused elevation o f the optimum temperature and spread of the pH range for enzyme reacti on. The maximum degree of hydrolysis (73%) based on the water-soluble substrate from the waste newspapers was similar to that in the case of using cellulose powder as a starting material. The primary hydrolyzat e contained much cello-oligosaccharide (G(2)-G(5)) with glucose and a little hemicellulose sugar, but, after deacetylation and secondary hyd rolysis, glucose comprised 73% of the total hydrolyzate.