K. Tsukahara et C. Kimura, PHOTOINDUCED ELECTRON-TRANSFER REACTION BETWEEN CYTOCHROME-C AND ZINCMYOGLOBIN MODIFIED WITH DIETHYLENETRIAMINEPENTAACETIC ACID, Journal of electroanalytical chemistry [1992], 438(1-2), 1997, pp. 67-70
The kinetics and mechanism of the photoinduced electron-transfer react
ion between cytochrome c and zinc-substituted myoglobin, the latter of
which lysine residue was modified with diethylenetriaminepentaacetic
acid, were studied in an aqueous solution at 25 degrees C, pH = 7.0 (a
10 mM phosphate buffer) and ionic strengths from 0.02 to 0.32 M (NaCl
). The excited triplet-state of the modified zinc myoglobin was effici
ently quenched by the oxidized cytochrome c within a diprotein complex
. Both the intracomplex electron-transfer quenching rate constant and
the binding constant of the diprotein complex decreased with increasin
g ionic strengths. (C) 1997 Elsevier Science S.A.