CONSTRUCTION OF A PANEL OF CANINE-RODENT HYBRID CELL-LINES FOR USE INPARTITIONING OF THE CANINE GENOME

We have constructed a collection of canine-rodent microcell hybrid cel l lines by fusion of canine fibroblast microcell donors with immortali zed rodent recipient cells. Characterization of the hybrid cell lines using a combination of fluorescence in situ hybridization and PCR anal ysis of canine microsatellite repeat sequences allowed selection of a panel of hybrids in which most canine chromosomes are represented. App roximately 90% of genetic markers and genes that were tested could be assigned to 1 of 31 anonymous canine chromosome groups, based on commo n patterns of retention in the hybrid set. Many of these putative chro mosome groups have now been validated by linkage analysis. This panel of cell lines provides a tool for development of genetic, physical, an d comparative maps of the canine genome. (C) 1997 Academic Press.