GENOMIC STRUCTURE AND CHROMOSOMAL LOCALIZATION OF MOUSE CYCLIN G1 GENE

Mouse genomic DNA harboring the full coding sequence of cyclin G1 was cloned and analyzed. The locations of five coding exons and the intron -exon boundary sequences were found to be conserved between the mouse and the human genes. Two putative binding sites for the p53 tumor supp ressor gene product were found around the first exon: one was located in the 5' regulatory region, and the other was in the first intron. Th e mouse cyclin G1 gene was mapped to bands A5 to B1 of chromosomes 11 (11A5-B1) by FISH using genomic DNA clone as a biotinylated probe. The location of mouse cyclin G1 is syntenic to that of its human homologu e, which we previously mapped to 5q32-q34 of chromosome 5. An addition al faint signal was detected on chromosome 4 (4B1-C2), probably indica ting the presence of a cyclin G1-related gene or pseudogene in the mou se genome. (C) 1997 Academic Press.