SUBREGIONAL LOCALIZATION OF 21 CHROMOSOME-7-SPECIFIC EXPRESSED SEQUENCE TAGS (EST) BY FISH USING NEWLY IDENTIFIED YAC AND P1

Twenty-one putative chromosome 7-derived expressed sequence tags (ESTs ) identified 33 yeast artificial chromosomes (YACs) or P1 clones, whic h were then used as reagents for physical mapping. FISH mapping establ ished that the ESTs contained within these clones were distributed thr oughout chromosome 7, with all major cytogenetic bands represented, ex cept 7p13-p15, 7p11, 7q31.2, and 7q35. Each EST sequence identified at least one other sequence in publicly available databases (using searc h tools such as BLASTN, basic local alignment search tool), and many o f the ESTs identified cDNAs and several genomic DNA sequences. However , 7 ESTs did not identify highly significant matches (P < 1 x 10(-5)). Only one (EST01924-D7S2281E) failed to identify any other EST from th e dbEST homology searches. BLAST analysis identified at least five gen es from EST sequence comparisons: protein tyrosine phosphatase zeta (P TPRZ, also known as RPTPZ) (EST02092), which we had mapped to 7q31.3, in agreement with previous studies; cAMP-dependent protein kinase regu latory subunit bI (EST01644); rat integral membrane glycoprotein (EST0 0085); human IFNAR gene for interferon alpha/beta receptor (EST00817); and rat 14-3-3 protein gamma subtype (putative protein kinase C regul atory protein) (EST00762). These ESTs will help to develop the map of chromosome 7, which integrates physical, transcriptional, and cytogene tic data, as sell as to provide candidate disease genes for chromosome 7-specific disorders. (C) 1997 Academic Press.