ACCUMULATION KINETICS OF VIRAL GENE-PRODUCTS IN CAULIFLOWER MOSAIC-VIRUS-INFECTED TURNIP PROTOPLASTS

The expression of cauliflower mosaic virus (CaMV) genes was studied in a turnip protoplast system. Six CaMV-encoded gene products were detec ted in infected turnip protoplasts by means of Western blotting. The i nfected turnip protoplasts showed different patterns of protein accumu lation; e.g. an open reading frame (ORF) I-encoded movement protein, a n ORF V-encoded reverse transcriptase and an ORF VI-encoded posttransc riptional transactivator representing the early accumulated proteins, an ORF II-encoded aphid transmission factor and an ORF IV-encoded coat protein the late accumulated proteins and an ORF III-encoded DNA bind ing protein the intermediate protein. The results suggest that the exp ression of CaMV genes is differentially regulated.