ADP-GLUCOSE PYROPHOSPHORYLASE IS LOCALIZED TO BOTH THE CYTOPLASM AND PLASTIDS IN DEVELOPING PERICARP OF TOMATO FRUIT

The intracellular location of ADP-glucose pyrophosphorylase (AGP) in d eveloping pericarp of tomato (Lycopersicon esculentum Mill) has been i nvestigated by immunolocalization. With the use of a highly specific a nti-tomato fruit ACP antibody, the enzyme was localized in cytoplasm a s well as plastids at both the light and electron microscope levels. T he immunogold particles in plastids were localized in the stroma and a t the surface of the starch granule, whereas those in the cytoplasm oc curred in cluster-like patterns. Contrary to the fruit, the labeling i n tomato leaf cells occurred exclusively in the chloroplasts. These da ta demonstrate that ACP is localized to both the cytoplasm and plastid s in developing pericarp cells of tomato.