QUANTITATIVE STUDY OF COMPETITIVE-BINDING OF DRUGS TO PROTEIN BY MICRODIALYSIS HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

Authors
WANG HL ZOU HF ZHANG YK
Citation
Hl. Wang et al., QUANTITATIVE STUDY OF COMPETITIVE-BINDING OF DRUGS TO PROTEIN BY MICRODIALYSIS HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY, Analytical chemistry, 70(2), 1998, pp. 373-377
Citations number
16
Categorie Soggetti
Chemistry Analytical
Journal title
Analytical chemistry → ACNP
ISSN journal
00032700
Volume
70
Issue
2
Year of publication
1998
Pages
373 - 377
Database
ISI
SICI code
0003-2700(1998)70:2<373:QSOCOD>2.0.ZU;2-W
Abstract
A displacement equation describing competitive binding of drugs to pro tein in solution is derived and examined with four nonsteroidal anti-i nflammatory drugs and human serum albumin as model drugs and protein, respectively. Microdialysis/high-performance liquid chromatography was adopted to determine simultaneously the unbound solute and displacing agent in drug-protein solutions. The method is able to locate the bin ding site and determine affinity constants even up to 10(7) L/mol accu rately. A comparison of association constants determined by this metho d and from capacity factors on HSA-CSP is given.