PRIMARY STRUCTURE AND FUNCTIONAL-ACTIVITY OF THE BACILLUS-SUBTILIS GENE RIBC

Authors
GUSAROV II KRENEVA RA RYBAK KV PODCHERNYAEV DA IOMANTAS YV KOLIBABA LG POLANUER BM KOZLOV YI PERUMOV DA
Citation
Ii. Gusarov et al., PRIMARY STRUCTURE AND FUNCTIONAL-ACTIVITY OF THE BACILLUS-SUBTILIS GENE RIBC, Molecular biology, 31(5), 1997, pp. 698-703
Citations number
22
Categorie Soggetti
Biology
Journal title
Molecular biology → ACNP
ISSN journal
00268933
Volume
31
Issue
5
Year of publication
1997
Pages
698 - 703
Database
ISI
SICI code
0026-8933(1997)31:5<698:PSAFOT>2.0.ZU;2-I
Abstract
The ribC gene controlling the riboflavin operon in Bacillus subtilis w as cloned by the resistance to a riboflavin analog 7,8-dimethyl-10-(O- methylcetoxime)-isoalloxazine. The homology was revealed with the ribo flavin kinase/FAD synthase gene from Corynebacrerium ammoniagenes and Escherichia coli. Riboflavin kinase/FAD synthase activity was detected in cell-free extracts, thus demonstrating that the ribC gene codes fo r a bifunctional enzyme producing FAD from riboflavin via FMN.