Mixed bacterial culture consisting of three different strains of Micro
coccus sp. (AG 36, AG 94 and AG 98) and two strains of Pseudomonas sp.
(AG 7 and AG 52) and its individual components was passed through a s
and column and 25.5-92 % of cell dry mass was found to be retained (ad
sorbed) on it. Incubation of sand soaked in mineral medium containing
glucose as a sole carbon source resulted in formation of a biofilm wit
h 1.2-25-fold increase in biomass. A 61 % degradation of malathion by
the mixed culture biofilm could be achieved in 4 d.