NESTED GENETIC BIT ANALYSIS (N-GBA) FOR MUTATION DETECTION IN THE P53TUMOR-SUPPRESSOR GENE

There is a growing and significant demand for reliable, simple and sen sitive methods for repeated scanning of a given gene or gene fragment for detection and characterization of mutations. Solid-phase sequencin g by single base primer extension of nested GBA(TM) primers on miniatu rized DNA arrays can be used to effectively scan targeted sequences fo r missense, insertion and deletion mutations, This paper describes the use of N-GBA arrays designed to scan the sequence of a 33 base region of exon 8 of the p53 gene (codons 272-282) encompassing a hot spot fo r mutations associated with the development of cancer, Synthetic DNA t emplates containing various missense, insertion and deletion mutations , as well as DNA prepared from pancreatic and biliary tumor cells, wer e genotyped using the exon 8 arrays.