PREDICTING THE IN-VITRO TOXICITY OF SYNTHETIC BETA-AMYLOID-(1-40)

The in vitro toxicity of synthetic beta-amyloid (beta A4) is variable and unpredictable, limiting its use as a research tool. This study des cribes a method using Congo red (CR) to predict the in vitro toxicity of beta A4 solutions. Histopathologically, CR is used to stain the neu ritic, beta A4-containing plaques, one of the hallmarks of Alzheimer's disease. In this study, synthetic beta A4 solutions were incubated wi th CR at a molar ratio of 1.0:2.5. The solutions were centrifuged and the absorbance of the supernatants were measured. Predictions of nonto xicity correlated with absorbance readings near zero. Toxicity was eva luated relative to control cells (vehicle only), using a hemocytometer to count PC-12 cells that excluded trypan blue. The positive predicti ve value of the test was 78% and the negative predictive value was 100 %. To use this test, the toxic concentration(s) of beta A4 must first be established empirically. Then, the CR test can be used to evaluate the potential toxicity of beta A4 solutions ar similar concentrations. Thus, this test can be used under a variety of laboratory circumstanc es. (C) 1997 Elsevier Science Inc.