METHODS FOR THE CHARACTERIZATION OF PHOSPHATASE-STABILIZED 2-5A-ANTISENSE CHIMERAS

Citation
Mr. Player et al., METHODS FOR THE CHARACTERIZATION OF PHOSPHATASE-STABILIZED 2-5A-ANTISENSE CHIMERAS, Bioconjugate chemistry, 9(1), 1998, pp. 137-142
Citations number
22
Categorie Soggetti
Chemistry Inorganic & Nuclear",Biology,"Biochemical Research Methods
Journal title
ISSN journal
10431802
Volume
9
Issue
1
Year of publication
1998
Pages
137 - 142
Database
ISI
SICI code
1043-1802(1998)9:1<137:MFTCOP>2.0.ZU;2-9
Abstract
We have developed chromatographic and spectrophotometric assays for de termining the degree of thiolation in phosphatase-resistant 5'-monothi ophosphate-capped 2-5A-antisense chimeras. Concomitantly, we have expl ored the reactivity of this 5'-monophosphorothioate moiety with report er reagents such as 5-iodoacetomidofluorescein and 5,5'-dithiobis(2-ni trobenzoic acid). On the basis of these reactions, analyses for 5'-mon othiophosphate-functionalized 2-5A-antisense chimeras were made possib le. Kinetic experiments demonstrated that oligonucleotide backbone neg ative charge could retard mixed disulfide formation in the reaction of 5,5'-dithiobis(2-nitrobenzoic acid) with 5'-monothiophosphorylated 2- 5A-antisense chimeras.